Elevated advanced oxidation protein products (AOPPs) indicate metabolic risk in severely obese children

Codoñer-Franch, P.; Tavárez-Alonso, S.; Murria-Estal, R.; Tortajada-Girbés, M.; Simó-Jordá, R.; Alonso-Iglesias, E.

doi:10.1016/j.numecd.2010.06.002

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Elevated advanced oxidation protein products (AOPPs) indicate metabolic risk in severely obese children

P. Codoñer-Franch
- Department of Pediatrics, Dr. Peset University Hospital, Avda Gaspar Aguilar 90, 46017 Valencia, Spain
- Department of Pediatrics, Obstetrics and Gynecology, University of Valencia, Avda Blasco Ibañez 15, 46010 Valencia, Spain
- Corresponding author at: Department of Pediatrics, Dr. Peset University Hospital, Avda Gaspar Aguilar 90, 46017 Valencia, Spain. Tel.: +34 961622389; fax: +34 963864815.
S. Tavárez-Alonso
- Department of Biochemistry and Molecular Biology, University of Valencia, Avda Blasco Ibañez 15, 46010 Valencia, Spain
R. Murria-Estal
- Clinical Biochemistry Laboratory, Dr. Peset University Hospital, Avda Gaspar Aguilar 90, 46017 Valencia, Spain
M. Tortajada-Girbés
- Department of Pediatrics, Dr. Peset University Hospital, Avda Gaspar Aguilar 90, 46017 Valencia, Spain
R. Simó-Jordá
- Department of Pediatrics, Dr. Peset University Hospital, Avda Gaspar Aguilar 90, 46017 Valencia, Spain
E. Alonso-Iglesias
- Department of Biochemistry and Molecular Biology, University of Valencia, Avda Blasco Ibañez 15, 46010 Valencia, Spain

Received 21 January 2010; received in revised form 25 April 2010; accepted 3 June 2010. published online 13 August 2010.
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Abstract

Background and aims

The assessment of oxidative stress may aid in the identification of subsequent metabolic risk in obese children. The objective of this study was to determine whether the plasma level of advanced oxidation protein products, analyzed with a recently proposed modified assay that involves a delipidation step (mAOPPs), was related to metabolic risk factors (MRFs) in severely obese children.

Methods and results

The plasma levels of mAOPPs were determined by spectrophotometry in 54 severely obese and 44 healthy children. We also measured lipid peroxidation biomarkers (thiobarbituric acid-reactive substances, malondialdehyde, and 8-isoprotane F_2α) and sulfhydryl groups, a marker of antioxidant defense. Protein oxidation and lipid peroxidation markers were higher and sulfhydryl levels were lower in obese children compared with controls. Taking metabolic risk into account, obese children were subdivided according to the cutoff point (53.2 μmol/L) obtained for their mAOPPs values from the ROC curve. Anthropometric measures and the existence of hypertension did not differ between groups. The presence of dyslipidemia and insulin resistance was significantly higher in the group with higher mAOPPs levels. The highest levels of mAOPPs were found in the children with ≥3 MRFs. The level of mAOPPs was positively correlated with triglycerides and negatively correlated with high-density lipoprotein cholesterol. There was no correlation of this marker of protein oxidation with biomarkers of lipid peroxidation.

Conclusion

The determination of mAOPPs in delipidated plasma is an easy way to evaluate protein oxidation. It may be useful in severely obese children for better cardiovascular risk assessment.

Keywords: Advanced oxidation protein products, Childhood obesity, Insulin resistance, Metabolic risk factors, Oxidative stress

Abbreviations: AOPPs, advanced oxidation protein products, mAOPPs, modified advanced oxidation protein products, BMI, body mass index, BP, blood pressure, FGIR, fasting glucose-to-insulin ratio, HDL-C, high-density lipoprotein cholesterol, HOMA-IR, homeostasis model assessment index, MRFs, metabolic risk factors, -SH groups, sulfhydryl groups, TG, triglycerides, WC, waist circumference