Summary
Aim
Of the many biological targets of oxidative stress, lipids are the most involved class
of biomolecules. Lipid oxidation gives rise to a number of secondary products. Malondialdehyde
(MDA) is the principal and most studied product of polyunsaturated fatty acid peroxidation.
This aldehyde is a highly toxic molecule and should be considered as more than just
a marker of lipid peroxidation. Its interaction with DNA and proteins has often been
referred to as potentially mutagenic and atherogenic. This review is intended to briefly
describe the physiological origin of MDA, to highlight its toxicity, describe and
comment on the most recent methods of detection and discuss its occurrence and significance
in pathology.
Data synthesis
In vivo origin as well as reactivity and consequent toxicity of MDA are reviewed.
The most recent and improved procedures for the evaluation of MDA in biological fluids
are described and discussed. The evidence of the occurrence of increased MDA levels
in pathology is described.
Conclusions
In the assessment of MDA, the most common methods of detection are insufficiently
sensitive and disturbed by interference coming from related species or overestimation
derived from stressing analysis conditions. Moreover, no recent nutritional or medical
trials report the use of one of the new and more reliable methods, some of which are
undoubtedly accessible to virtually all the laboratories provided with a common HPLC
or a spectrofluorimeter.
Keywords
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Article info
Publication history
Accepted:
May 5,
2005
Received in revised form:
May 2,
2005
Received:
July 21,
2004
Identification
Copyright
© 2005 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
