Abstract
Glutathione peroxidase-1 (GPx-1) is an endogenous anti-oxidant enzyme. The T allele
of the GPx-1 rs1050450 (C > T) gene variant is associated with reduced enzyme activity. Our aim was to examine
the association between this gene variant and peripheral neuropathy in two cross-sectional
samples of subjects with diabetes: (i) 773 Caucasian subjects were genotyped from
the UCL Diabetes and Cardiovascular disease Study (UDACS) and (ii) 382 Caucasian subjects
from the Ealing Diabetes Study (EDS). Peripheral neuropathy status (and oxidised-LDL
[Ox-LDL:LDL] and plasma Total Ant-ioxidant Status [TAOS] in UDACS), were analysed
in relation to genotype. We observed that: (i) In UDACS, the odds ratio (OR) for peripheral
neuropathy in the T allele carriers compared to the CC genotype was 1.61 [1.10–2.28],
p = 0.01. This remained significant after adjustment for other risk factors. Ox-LDL:LDL
ratio was significantly elevated in T allele carriers (CC vs. CT/TT: 16.3 ± 2.4 v
18.0 ± 2.9 U/mmol LDL, p = 0.02). (ii) In EDS, the OR for peripheral neuropathy in the T allele carriers compared
to the CC genotype was 1.95 [1.11–3.42], p = 0.02. This remained significant after adjustment for other risk factors. In conclusion,
we observed a significant association between the T allele and peripheral neuropathy
and LDL oxidation. This is the first paper to examine the rs1050450 variant in two samples of Caucasian subjects with diabetes. Prospective analysis
of the gene variant is required in diabetic and healthy cohorts with measured plasma
markers of oxidative stress to investigate the described association further.
Keywords
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Article info
Publication history
Published online: December 27, 2010
Accepted:
August 2,
2010
Received in revised form:
June 2,
2010
Received:
February 17,
2010
Identification
Copyright
© 2010 Elsevier B.V. Published by Elsevier Inc. All rights reserved.
