Research Article| Volume 25, ISSUE 1, P52-59, January 2015

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Docosahexaenoic acid-enriched canola oil increases adiponectin concentrations: A randomized crossover controlled intervention trial


      • DHA-rich canola oil increases adiponectin compared with PUFA from plant sources.
      • Anti-inflammatory effects of DHA may occur through down-regulation of IL1B.
      • MUFA-rich regular canola oil reduces hs-CRP compared with PUFA from plant sources.


      Background and aims

      Little is known about the effect of various dietary fatty acids on pro- and anti-inflammatory processes. We investigated the effect of 5 oils containing various amounts of alpha-linolenic acid (ALA), linoleic acid (LA), oleic acid (OA) and docosahexaenoic acid (DHA) on plasma inflammatory biomarkers and expression levels of key inflammatory genes and transcription factors in whole blood cells.

      Methods and results

      In a randomized, crossover controlled nutrition intervention, 114 adult men and women with abdominal obesity and at least one other criterion for the metabolic syndrome consumed 5 experimental isoenergetic diets for 4 weeks each, separated by 4-week washout periods. Each diet provided 60 g/3000 kcal of different oils: 1) control corn/safflower oil blend (CornSaff; LA-rich), 2) flax/safflower oil blend (FlaxSaff; ALA-rich), 3) conventional canola oil (Canola; OA-rich), 4) high oleic canola oil (CanolaOleic; highest OA content), 5) DHA-enriched high oleic canola oil (CanolaDHA; OA- and DHA-rich). Gene expression in whole blood cells was assessed in a subset of 62 subjects. CanolaDHA increased plasma adiponectin concentrations compared with the control CornSaff oil treatment (+4.5%, P = 0.04) and FlaxSaff (+6.9%, P = 0.0008). CanolaDHA also reduced relative expression levels of interleukin (IL)1B compared with CornSaff and Canola (−11% and −13%, respectively, both P = 0.03). High-sensitivity C-reactive protein concentrations were lower after Canola than after FlaxSaff (−17.8%, P = 0.047).


      DHA-enriched canola oil exerts anti-inflammatory effects compared with polyunsaturated fatty acids from plant sources.



      ALA (alpha-linolenic acid), BMI (body mass index), BP (blood pressure), CCL2 (chemokine (C–C motif) ligand 2), cDNA (complementary deoxyribonucleic acid), COMIT (Canola Oil Multicenter Intervention Trial), CRP (C-reactive protein), CV (coefficient of variation), Ct (cycle threshold), CVD (cardiovascular disease), DHA (docosahexaenoic acid), EPA (eicosapentaenoic acid), G6PD (glucose-6-phosphate dehydrogenase), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), HDL-C (high-density lipoprotein cholesterol), hs-CRP (high-sensitivity CRP), IDF (International Diabetes Federation), IL (interleukin), LA (linoleic acid), LDL-C (low-density lipoprotein cholesterol), MetSyn (metabolic syndrome), MUFA (monounsaturated fatty acids), NFKB1 (nuclear factor kappa-B subunit 1), NPR3 (natriuretic peptide receptor C), OA (oleic acid), PPAR (peroxisome proliferator-activated receptor), PPARA (PPAR alpha), PUFA (polyunsaturated fatty acids), RNA (ribonucleic acid), SD (standard deviation), SFA (saturated fatty acids), SREBF2 (sterol regulatory element-binding transcription factor 2), TG (triglycerides), TNF (tumor necrosis factor), TRAF3 (TNF receptor-associated factor 3)
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