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Modulation of gene expression profile following consumption of high-dairy products in subjects with hyperinsulinemia

  • Leila Khorraminezhad
    Affiliations
    Endocrinology and Nephrology Unit, CHU de Québec-Laval University Research Center, Quebec, Canada
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  • Iwona Rudkowska
    Correspondence
    Corresponding author. Endocrinology and Nephrology Unit, CHU de Québec Research Center, CHUL - 2705, boulevard. Laurier, Québec, Québec, G1V 4G2. Canada.
    Affiliations
    Endocrinology and Nephrology Unit, CHU de Québec-Laval University Research Center, Quebec, Canada

    Department of Kinesiology, Laval University, Québec (QC), Canada
    Search for articles by this author
Published:November 01, 2022DOI:https://doi.org/10.1016/j.numecd.2022.10.015

      Highlights

      • Regulation of 236 genes changed after high-dairy intake using microarray techniques.
      • Molecular pathways associated to glucose homeostasis were modified after high-dairy intake.
      • A potential protective effect of high dairy consumption was observed among individuals at risk of T2D.

      Abstract

      Background and aim

      Dysregulation of gene expression is associated to a higher risk of type 2 diabetes (T2D). Further, research indicates that dairy consumption may potentially affect gene expression.
      The aim of this study was to examine if genes and pathways associated with T2D are differentially changed in subjects with hyperinsulinemia after high dairy (HD) diet.

      Methods and results

      Ten obese patients with hyperinsulinemia who consumed HD (4 servings/day according to the Canadian Food Guide (2007)) for six weeks participated in this study. Before and after HD consumption, fasting blood samples were collected. Blood was taken in PAX-gene tubes and RNA was extracted and analyzed using Clariom S microarrays. Results indicated that 236 genes (137 up-regulated and 99 down-regulated; fold change (FC) ≥ ±1.2; p < 0.05) were expressed differentially between before and after HD intake. Genes related to pathways associated with insulin signaling and inflammation, such as olfactory receptor activity, G-protein-coupled receptors (GPCR), phosphatidylinositol-3-OHKinase (PI3K)/AKT2 (PI3K-AKT2), Ras signaling, Mitogen-Activated Protein Kinase (MAPK) were altered following HD.

      Conclusion

      Overall, results suggest a potential protective effect of HD intake in individuals at risk of T2D through modification of gene expression profiles.

      Registration number for clinical studies

      NCT02961179.

      Keywords

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